Diabetes Mellitus

Leading researchers and clinical investigators describe their best cutting-edge techniques for studying, at both the molecular and biochemical levels, the defects in insulin production and action associated with diabetes. Written in step-by-step detail to ensure ready reproducibility and robust results, these techniques allow investigators, both novices and those already active in the field, to study every major facet of insulin production and action. Each protocol includes an introduction to the technique, an explanation of its application, and a list of materials. Practical notes discuss how to avoid pitfalls, as well as how to adapt the methods to your own research.

ISBN	1588291480	Volumes	1
ISBN13	9781588291486 (What's this?)	Weight (grammes)	1060
Publisher	Humana Press Inc.	Published in	Totowa, NJ
Imprint	Humana Press Inc.	Series ISSN	83
Format	Hardback	Series title	Methods in Molecular Medicine
Publication date	04 Feb 2003	Height (mm)	229
Library of Congress	02032816	Width (mm)	152
DEWEY	616.46207	Spine width (mm)	15
DEWEY edition	DC21	Academic level	Undergraduate, Professional / Scholarly, Postgraduate
Pages	208

		Preface
		Contributors
1		Isolation of Islets of Langerhans from Rodent Pancreas by Colleen B. Kelly and Libby A. Blair and John A. Corbett and Anna L. Scarim
2		Purification of Rat Pancreatic [beta]-Cells by Fluorescence-Activated Cell Sorting by Geert Stange and Mark Van De Casteele and Harry Heimberg
3		Assessment of Insulin Secretion in the Mouse by Marcela Brissova and Wendell E. Nicholson and Masakazu Shiota and Alvin C. Powers
4		Detection of Insulin Production by Immunohistochemistry by Susan C. Campbell and Wendy M. Macfarlane
5		Quantification of the Level of Insulin Gene Expression by Susan C. Campbell and Wendy M. Macfarlane
6		Chromatin Immunoprecipitation Using Isolated Islets of Langerhans by Marcelina Parrizas and Sylvia F. Boj and Reini F. Luco and Miguel Angel Maestro and Jorge Ferrer
7		Adenoviral Gene Transfer into [beta]-Cell Lines by Amber L. Mosley and Sabire Ozcan
8		Utilization of NOD Mice in the Study of Type 1 Diabetes by Dennis G. Karounos and Susan E. Goes
9		Introduction of DNA into 3T3-L1 Adipocytes by Electroporation by Shuichi Okada and Masatomo Mori and Jeffrey E. Pessin
10		Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytes by Jaganathan Lakshmanan and Jeffrey S. Elmendorf and Sabire Ozcan
11		Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytes by Jeffrey S. Elmendorf
12		Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assay by John B. Knight and Ann Louise Olson
13		Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates by Eileen L. Whiteman and Morris J. Birnbaum
14		Measuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase Activity by Li-Ping Wang and Scott A. Summers
15		Assaying AKT/Protein Kinase B Activity by Kyle L. Hoehn and Scott A. Summers
		More...

"This book provides all the critical tools needed for the productive study of diabetes mellitus today." - Clinical Laboratory International "Each chapter/protocol is well referenced and the book is indexed. Because Diabetes Mellitus: Methods and Protocols provides all the critical tools needed for the productive study of diabetes mellitus, it is a necessary purchase for libraries supporting basic research programs that study the molecular and biochemical bases of diabetes mellitus." -E-STREAMS

Be the first to write a customer review

Methods and Protocols

Buying options