HPLC Columns

An in-depth guide to HPLC column technology High-performance liquid chromatography and its derivative techniques have become the dominant analytical separation tools in the pharmaceutical, chemical, and food industries; environmental laboratories; and therapeutic drug monitoring. Although the column is the heart of the HPLC instrument and essential to its success, until now, no book has focused on the theory and practice of column technology. HPLC Columns provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and academician alike. Along with a comprehensive discussion of the chemical and physical processes of the HPLC column, it includes fundamental principles, separation mechanisms and available technologies, column selection criteria, and special techniques. Special features include: Comprehensive overview of state-of-the-art HPLC column technology Explanation of the underlying principles of HPLC columns Methods for selecting columns Practical advice on using and applying columns, including examples Section by M. Zoubair El Fallah on methods development Special techniques, including preparative chromatography, continuous chromatography, and the simulated moving bed Troubleshooting section HPLC Columns helps laboratory practitioners make better choices in column selection, methods development, and troubleshooting: it is also an excellent textbook for graduate-level courses and HPLC short courses.

ISBN 0471190373   Pages 416 ISBN13 9780471190370 (What's this?)   Volumes 1 Publisher John Wiley and Sons Ltd   Weight (grammes) 696 Imprint John Wiley & Sons Inc   Published in New York Format Hardback   Height (mm) 239 Publication date 01 Aug 1997   Width (mm) 163 Library of Congress QD79.C454N   Spine width (mm) 24 DEWEY 543.0894   Academic level Undergraduate, Postgraduate, Professional / Scholarly DEWEY edition DC21

		Preface
1		Introduction		1
2		Theory of Chromatography		6
3		Column Design		41
4		Physical Properties of HPLC Packings		81
5		Column Packing and Testing		93
6		Column Chemistry		106
7		Column Selection		130
8		Size-Exclusion Chromatography		140
9		Normal-Phase Chromatography		164
10		Reversed-Phase Chromatography		183
11		Hydrophilic Interaction Chromatography		217
12		Ion-Exchange Chromatography		224
13		Hydrophobic Interaction Chromatography		250
14		Special Techniques		261
15		Preparative Chromatography		276
16		HPLC Methods Development		296
17		Column Maintenance		342
App		Teaching Column Chromatography		380
		List of Symbols		383
		Index		389

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