Immunology of Infection (Vol 32)

"Immunology of Infection, 2nd Edition", edited by two leading experts in the field, presents the most appropriate up-to-date experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the Microbiologist interested in analysing host-pathogen relationships, this volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology and parasitology. This new edition of "Immunology of Infection" provides ready-to-use 'recipes', and the latest emerging techniques as well as novel approaches to the tried and tested, established methods included in the successful first edition. "Methods in Microbiology" is the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, "Methods in Microbiology" will continue to provide you with tried and tested, cutting-edge protocols to directly benefit your research. This book includes techniques for genome-wide expression profiling of both the pathogen and host and of the host response to infection. It provides a cytometric analysis of cytokine secretion by immune cells. It describes tetramer technology for the quantitative analysis of antigen specific T cell responses. It offers an analysis of host cells and pathogens involved in the host-microbe interplay. It covers techniques useful for the analysis of human and murine systems. It includes techniques for the prediction and determination of MHC ligands and T cell epitopes. It covers the fundamentals and practice of DNA vaccines. It describes methods for the isolation and propagation of human dendritic cells.

ISBN 0125215320   Volumes 1 ISBN13 9780125215329 (What's this?)   Weight (grammes) 1670 Publisher Elsevier Science & Technology   Published in Oxford Imprint Academic Press Inc   Series editor Dougan, Gordon (Imperial College of Science, Technology and Medicine,, Dougon, Gordon, Dougan, Gordo Format Hardback   Series ISSN 32 Publication date 25 Nov 2002   Series title Methods in Microbiology S. Library of Congress QR   Height (mm) 189 DEWEY 616.047   Width (mm) 246 DEWEY edition DC21   Academic level Professional / Scholarly Pages 824

		Contributors
		Preface to the First Edition
		Preface to the Second Edition
		Introduction: The immune response to infectious agents by S. H. E. Kaufmann and D. Kabelitz		1
I		General Methods
1		Phenotyping and separation of leukocyte populations based on affinity labelling by A. Scheffold and M. Assenmacher and A. Radbruch		23
2		Cytometric cytokine secretion assay: detection and isolation of cytokine-secreting T cells by M. Assenmacher and A. Scheffold and A. Radbruch		59
3		Measurement of cellular proliferation by K. Pechhold and N. Craighead and D. Wesch and D. Kabelitz		77
4		Use of bioinformatics to predict MHC ligands and T-cell epitopes: application to epitope-driven vaccine design by A. S. De Groot and H. Sbai and B. Martin and J. A. Berzofsky		99
5		The use of tetramers in the quantitative analysis of T-cell responses by G. M. A. Gillespie and V. Appay and S. L. Rowland-Jones and A. J. McMichael		125
6		Using microarrays for studying the host transcriptional response to microbial infection by D. Yowe and D. Farlow and J. Lora and S. Drabic and J.-C. Gutierrez-Ramos		157
7		Genome-wide expression profiling of intracellular bacteria: the interaction of Mycobacterium tuberculosis with macrophages by S. Ehrt and M. I. Voskuil and G. K. Schoolnik and D. Schnappinger		169
II		Murine Models
1		Management of immunocompromised and infected animals by H. Mossmann and W. Nicklas and H. J. Hedrich		183
2		In vitro analysis
2.1		Isolation and preparation of lymphocytes from infected animals for in vitro analysis by C. J. Czuprynski and J. F. Brown		233
2.2		Isolation and development of murine T-lymphocyte hybridomas and clonal cell lines by C. Reardon and M. Lahn		249
2.3		Killer cell assays by D. H. Busch and E. G. Pamer		281
		More...

Praise for the first edition: "This is a useful compendium of methods and techniques for those wishing to examine the immune responses to infections in vitro and in vivo." "The book is clearly presented, packed with protocols, and well illustrated" -Microbiology Today "...it represents a valuable addition to the microbiologists' reference source" -Immunology Today Praise for the Series: "An invaluable resource for the detailed analysis of cellular gene function and genome architecture." -NATURE

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