Immunology of Infection (v. 25)

Established for almost 30 years, "Methods in Microbiology" is the most prestigious series devoted to techniques and methodology in the field. Now totally revamped, revitalized, with a new format and expanded scope, "Methods in Microbiology" will continue to provide you with tried and tested, cutting-edge protocols to directly benefit your research. "Immunology of Infection", edited by two of the foremost figures in the field, presents the most appropriate, up-to-date techniques in the detail you require. The layout is structured for ease of reference, and the volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology, and parasitology. The new volume provides a carefully selected collection of immunological techniques for the microbiologist wishing to study host-pathogen relationships in vivo and in vitro. This multi-authored book has succeeded in bringing together experts from various fields of molecular and cellular immunology who provide ready-to-use recipes for the ex vivo and in vitro analysis of anti-infective immunity. It: focuses on the methods most useful for the microbiologist interested in analysing host-pathogen relationships; includes ready-to-use, tried and tested recipes; contains lists of suppliers provided as appendices to each chapter; covers techniques useful for the analysis of human and murine cells; includes techniques for the prediction and determination of MHC ligands and T cell epitopes; describes the art and science of DNA vaccines; covers essential methods for measuring human cytokine responses, as well as isolation and propagation of dendritic cells.

ISBN 0125215282   Part volume Immunology of Infection ISBN13 9780125215282 (What's this?)   Volumes 001 Publisher Elsevier Science & Technology   Weight (grammes) 1298 Imprint Academic Press Inc   Published in Oxford Format Hardback   Series editor Dougan, Gordon (Wolfson Laboratories, Imperial College of Science,, et al Publication date 20 Apr 1998   Series ISSN 25 Library of Congress QR   Series title Methods in Microbiology S. DEWEY 579   Height (mm) 244 DEWEY edition DC21   Width (mm) 172 Pages 705   Academic level Professional / Scholarly

		Contributors
		Preface
		Introduction: The immune response to infectious agents by S. H. E. Kaufmann and D. Kabelitz		1
I		General Methods
1		Phenotyping and separation of leukocyte populations based on affinity labelling by A. Scheffold and A. Radbruch		23
2		Measurement of cellular proliferation by K. Pechhold and D. Kabelitz		59
3		Prediction and determination of MHC ligands and T-cell epitopes by A. S. De Groot and B. M. Jesdale and J. A. Berzofsky		79
II		Murine Models
1		Management of immunocompromised and infected animals by H. Mossmann and W. Nicklas and H. J. Hedrich		109
2		In vitro analysis
2.1		Isolation and preparation of lymphocytes from infected animals by C. J. Czuprynski and J. F. Brown		189
2.2		Establishment of murine T-cell lines and clones, hybridomas and transfectomas by W. Born and C. Cady and M. Lahn		205
2.3		Killer cell assays by D. H. Busch and E. G. Pamer		237
2.4		Measuring cytokine responses by ELISA, ELISPOT and RT-PCR methods by K. Fujihashi and J. L. VanCott and J. R. McGhee		257
2.5		Isolation of and measuring the function of professional phagocytes: Murine macrophages by R. Haworth and S. Gordon		287
3		In vivo analysis
3.1		The immune response in mice challenged with bacterial infections by M. E. A. Mielke and I. B. Autenrieth and T. K. Held		313
3.2		Measuring immune responses by S. Ehlers and U. Seitzer		365
4		Specific models
4.1		Murine models of tuberculosis by A. D. Roberts and J. T. Belisle and A. M. Cooper		389
4.2		The leishmaniasis model by P. Kropf and I. Muller and K. Brunson		419
		More...

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