Neural Cell Transplantation

In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In Neural Cell Transplantation: Methods and Protocols, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can be based. Written in the highly successful Methods in Molecular Biology(t) series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and Notes sections, which examine tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Neural Cell Transplantation: Methods and Protocols provides the most thorough and essential protocols that will allow new generations of neuroscientists to enter and contribute to this uniquely inspiring field.

ISBN 160327930X   Volumes 1 ISBN13 9781603279307 (What's this?)   Weight (grammes) 703 Publisher Humana Press Inc.   Published in Totowa, NJ Imprint Humana Press Inc.   Series ISSN 549 Format Hardback   Series title Methods in Molecular Biology Publication date 28 May 2009   Height (mm) 260 DEWEY 617.480592   Width (mm) 193 DEWEY edition DC22   Spine width (mm) 18 Pages 240   Academic level Professional / Scholarly

Pt. I		Introductory Reviews
1		Neural Transplantation and Stem Cells by Mahendra S. Rao and Mohan C. Vemuri		3
2		Adult Stem Cells for the Treatment of Neurological Disease by C. M. Rice and N. J. Scolding		17
3		Human Trials for Neurodegenerative Disease by Claire M. Kelly and O. J. Handley and A. E. Rosser		33
Pt. II		Practial/Methodological Chapters
4		Differentiation of Neuroepithelia from Human Embryonic Stem Cells by Xiaofeng Xia and Su-Chun Zhang		51
5		Derivation of High-Purity Oligodendroglial Progenitors by Maya N. Hatch and Gabriel Nistor and Hans S. Keirstead		59
6		Flow Cytometric Characterization of Neural Precursor Cells and Their Progeny by Preethi Eldi and Rodney L. Rietze		77
7		Isolation, Expansion, and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells Using the Neurosphere Assay by Loic P. Deleyrolle and Brent A. Reynolds		91
8		Human Mesenchymal Stem Cell Culture for Neural Transplantation by David Gordon and Neil J. Scolding		103
9		Umbilical Cord Blood Cells by Jennifer D. Newcomb and Alison E. Willing and Paul R. Sanberg		119
10		Animal Models of Neurodegenerative Diseases by Wendy Phillips and Andrew Michell and Harald Pruess and Roger A. Barker		137
11		Animal Models of Multiple Sclerosis by Roberto Furlan and Carmela Cuomo and Gianvito Martino		157
12		Transplantation of Oligodendrocyte Progenitor Cells in Animal Models of Leukodystrophies by Yoichi Kondo and Ian D. Duncan		175
13		A Rat Middle Cerebral Artery Occlusion Model and Intravenous Cellular Delivery by Masanori Sasaki and Osamu Honmou and Jeffery D. Kocsis		187
14		MR Tracking of Stem Cells in Living Recipients by Eva Sykova and Pavla Jendelova and Vit Herynek		197
15		Identifying Neural Progenitor Cells in the Adult Brain by Stephen Kelly and Maeve Caldwell and Matthew P. Keasey and Jessica A. Cooke and James B. Uney		217
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From the reviews: "In this book, the authors discuss the current understanding of the management of degenerative diseases of the CNS. ! Neurologists, neurosurgeons, neurophysiologists, and neuroscientists are the intended audience. ! the book flows easily and describes the most common research and technical protocols associated with human stem cell handling and separation. It is a well-referenced and illustrated book and worth its value for most labs ! . a good book for neuroscientists who are directly involved in the separation and production of human stem cells." (Celso Agner, Doody's Review Service, December, 2009)

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