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How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99 per cent success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: Peptide fragmentation and interpretation of product ion spectra Basic polyacrylamide gel electrophoresis Preparation of protein digests for sequencing experiments Mass spectrometric analysis using capillary liquid chromatography Techniques for protein identification by database searches Characterization of modified peptides using tandem mass spectrometry And much more
| ISBN | 0471322490 | | Volumes | 1 | | ISBN13 | 9780471322498
(What's this?) | | Weight (grammes) | 552 | | Publisher | John Wiley and Sons Ltd | | Published in | New York | | Imprint | John Wiley & Sons Inc | | Series title | Wiley-Interscience Series on Mass Spectrometry | | Format | Hardback | | Height (mm) | 236 | | Publication date | 09 Oct 2000 | | Width (mm) | 161 | | Library of Congress | QP551.K495 | | Spine width (mm) | 21 | | DEWEY | 572.85 | | Academic level | Professional / Scholarly | | DEWEY edition | DC21 | | Alternative ISBN | 9780471721987 | | Pages | 320 | |
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| | | Series Preface | | | | | | Preface | | | | Ch. 1 | | An Introduction to Protein Sequencing Using Tandem Mass Spectrometry | | 1 | | Ch. 2 | | The Primary Structure of Proteins and a Historical Overview of Protein Sequencing | | 6 | | Ch. 3 | | Fundamental Mass Spectrometry | | 29 | | Ch. 4 | | Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra | | 64 | | Ch. 5 | | Basic Polyacrylamide Gel Electrophoresis | | 117 | | Ch. 6 | | The Preparation of Protein Digests for Mass Spectrometric Sequencing Experiments | | 147 | | Ch. 7 | | Mass Spectrometric Analysis of Tryptic Digests | | 166 | | Ch. 8 | | Protein Identification by Database Searching | | 207 | | Ch. 9 | | Sequence Analysis of Novel Proteins | | 238 | | Ch. 10 | | The Characterization of Post-Translationally Modified Proteins Using Tandem Mass Spectrometry | | 269 | | | | Index | | 295 |
"[the authors] present...laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods..." (SciTech Book News Vol. 25, No. 2 June 2001) "...an informative, well-written and well-illustrated book with a large number of useful references..." (Spectroscopyeurope.com) "...a well-written, highly illustrative book...upper-level college students and graduate students interested in proteomics should benefit from this concise yet practical book, as would scientists making a transition into the field." (Journal of Proteome Research, Vol. 1, No. 1, January/February 2002) "...the book does a creditable job of making detailed lab protocols accessible..." (The Analyst, September 2002) "...very impressive by its content and its clarity..." (Biochemie, Vol.84, No.4, 2002)  Be the first to write a customer review
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