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Experts present methods and protocols essential for understanding parasites at the molecular level. The protocols cover culturing techniques for the major experimental organisms, methods for isolating and processing nucleic acids and proteins, PCR-based protocols for parasite identification, gene isolation and mutation, antibody-based procedures, chromosome and epitope mapping, flow cytometry, RNA sequencing, and parasite transformation.
| ISBN | 0896032396 | | Volumes | 1 | | ISBN13 | 9780896032392
(What's this?) | | Weight (grammes) | 679 | | Publisher | Humana Press Inc. | | Published in | Totowa, NJ | | Imprint | Humana Press Inc. | | Series editor | Walker, J.M., Walker, J.M., Walker, J.M. | | Format | Paperback | | Series ISSN | 21 | | Publication date | 11 Aug 1993 | | Series title | Methods in Molecular Biology | | Non-book description | xiv, 470 p. : | | Height (mm) | 234 | | Library of Congress | 9308540 | | Width (mm) | 156 | | DEWEY | 574.52490724 | | Spine width (mm) | 24 | | DEWEY edition | DC20 | | Academic level | Undergraduate, Professional / Scholarly, Postgraduate | | Pages | 488 | |
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| | | Preface | | | | | | Contributors | | | | Ch. 1 | | Culturing and Biological Cloning of Trypanosoma brucei by Mark Carrington | | 1 | | Ch. 2 | | Culturing and Biological Cloning of Trypanosoma cruzi by Michael A. Miles | | 15 | | Ch. 3 | | In Vitro Cultivation and Biological Cloning of Leishmania by David A. Evans | | 29 | | Ch. 4 | | Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages) Suitable for Large-Scale Preparations by Martin Read and John E. Hyde | | 43 | | Ch. 5 | | Synchronization and Cloning of Malaria Parasites by David Walliker and Geoffrey Beale | | 57 | | Ch. 6 | | The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies by Richard Carter and Lisa Ranford-Cartwright and Pietro Alano | | 67 | | Ch. 7 | | The Culture of Schistosoma mansoni and Production of Life Cycle Stages by Fiona Hackett | | 89 | | Ch. 8 | | Preparation of DNA and RNA from Trypanosoma brucei by Mark Carrington | | 101 | | Ch. 9 | | Isolation of RNA and DNA from Trypanosoma cruzi by Frank Ashall | | 113 | | Ch. 10 | | Isolation of DNA and RNA from Leishmania by John M. Kelly | | 123 | | Ch. 11 | | The Extraction and Purification of DNA and RNA from In Vitro Cultures of the Malaria Parasite Plasmodium falciparum by John E. Hyde and Martin Read | | 133 | | Ch. 12 | | Extraction and Purification of Nucleic Acids from Schistosomes by Simon A. Jeffs and Andrew J. G. Simpson | | 145 | | Ch. 13 | | Application of DNA and Whole Organisms to Filter Supports for DNA Probe Analysis: Dot, Slot, and Touch Blotting by Margaret A. Hughes and Julian M. Crampton | | 155 | | Ch. 14 | | The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification by Margaret A. Hughes and Julian M. Crampton | | 169 | | Ch. 15 | | Analysis of the DNA of Parasitic Protozoa by Flow Cytometry by James A. Dvorak | | 191 | | | More... | | | | | | Index | | 459 |
...well-written and thoughtfully assembled...will be welcomed by recent arrivals...and regularly utilized by instructors of future investigators. - Analytical Biochemistry  Be the first to write a customer review
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