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The past decade has seen not only an explosive increase of interest in mycorrhiza as evidenced by the surge of papers published, but also, the development of a greater awareness amongst plant physiologists, ecologists, and microbiologists that the mycorrhizal condition is the norm rather than the exception. Through this same period, new technologies (eg. NMR, RFLP, DNA mediated transformation, immunocytochemistry), which are readily applicable to mycorrhizal research, have become widely available. This two volume text presents an up-to-date collection of laboratory techniques that can be routinely used in mycorrhizal research. The first volume emphasizes a description of techniques applicable to ectomycorrhizal and ericoid systems, while the second stresses vesicular-arbuscular systems. Each contains a mix of papers, written by experts, which emphasize techniques, but which also include reviews of particular topics such as carbon metabolism, nitrogen metabolism, and applied aspects. The methods whereby, and the extent to which, mychorrhizal inoculation can be used to facilitate revegetation, as well as increases of agricultural or forest productivity are also described.
| ISBN | 0125214901 | | Pages | 896 | | ISBN13 | 9780125214902
(What's this?) | | Volumes | 2 Spiral bound items | | Publisher | Elsevier Science & Technology | | Weight (grammes) | 1320 | | Imprint | Academic Press Inc | | Published in | Oxford | | Format | Spiral / Comb Bound | | Height (mm) | 235 | | Publication date | 25 Apr 1994 | | Width (mm) | 171 | | DEWEY | 576.078 | | Spine width (mm) | 51 | | DEWEY edition | DC20 | | Academic level | Undergraduate, Postgraduate, Professional / Scholarly |
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| | | Contributors | | | | | | Preface | | | | 1 | | Introduction: The State of the Art by J. L. Harley | | 1 | | 2 | | Characterization of Ectomycorrhiza by R. Agerer | | 25 | | 3 | | Techniques in Synthesizing Ectomycorrhiza by R. L. Peterson and P. Chakravarty | | 75 | | 4 | | Histochemistry of Ectomycorrhiza by R. L. Peterson | | 107 | | 5 | | Nuclear Magnetic Resonance Studies in Ectomycorrhizal Fungi by F. M. Martin | | 121 | | 6 | | Carbon Metabolism in Mycorrhiza by I. Jakobsen | | 149 | | 7 | | Enzymology of Nitrogen Assimilation in Mycorrhiza by I. Ahmad and J. A. Hellebust | | 181 | | 8 | | Techniques for the Study of Nitrogen Metabolism in Ectomycorrhiza by B. Botton and M. Chalot | | 203 | | 9 | | Ectomycorrhizal DNA: Isolation, RFLPs and Probe Hybridization by P. T. Rygiewicz and J. L. Armstrong | | 253 | | 10 | | Procedures and Prospects for DNA-Mediated Transformation of Ectomycorrhizal Fungi by P. A. Lemke and V. Barrett and R. K. Dixon | | 281 | | 11 | | Principles of Use of Radioisotopes in Mycorrhizal Studies by P. B. Tinker and M. D. Jones and D. M. Durall | | 295 | | 12 | | Techniques for Studying the Functional Aspects of Rhizomorphs of Wood-rotting Fungi: Some Possible Applications to Ectomycorrhiza by D. H. Jennings | | 309 | | 13 | | Structural and Ontogenic Study of Ectomycorrhizal Rhizomorphs by J. W. G. Cairney | | 331 | | 14 | | Fine-structural Analysis of Mycorrhizal Fungi and Root Systems: Negative Staining and Cryoelectron Microscopic Techniques by F. Mayer and M. Madkour and A. Nolte and A. Varma | | 341 | | 15 | | Epifluorescent Microscopy for Identification of Ectomycorrhiza by P. Cudlin | | 365 | | 16 | | Electron Energy Loss Spectroscopy and Imaging Techniques for Subcellular Localization of Elements of Mycorrhiza by I. Kottke | | 369 | | 17 | | Methods for Studying Nursery and Field Response of Trees to Specific Ectomycorrhiza by D. H. Marx and J. L. Ruehle and C. E. Cordell | | 383 | | | More... | | |
A Review of the Original Volumes"Mycorrhizal research has grown by leaps and bounds in the last decade...in recent years there has been an urgent need for a comprehensive and up-to-date source book of techniques; an easily available, detailed account of moderen, sophisticated methods routinely used in laboratories involved in mycorrhizal research. The present volumes meet this requirement well ... I applaud the editors for assembling an impeccable team of researchers, all of whom have contributed to the success of this volume which clearl provides more than mere laboratory procedures. Each author has attempted to provide a framework for the current status to his or her individual area, and outlined new and innovative methods along with the usage and significance of these methods. A vast amount of useful data generated from the application of the techniques reported is also shared. I see this volume as a tremendously valuable collection of methods in mycorrhizal research, seriously presented and snthesized ... This compilation is the best available."--PLANT SCIENCE BULLETIN  Be the first to write a customer review
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