Techniques of Molecular Biology

Tagu (research, INRA Rennes, France) and Moussard (biochemistry and molecular biology, U. of Franche-Comt , France) outline the theoretical principles of techniques of molecular biology in 57 short profiles written by researchers from France. Profiles detail the definition of genomes, vectors and cloning, the labeling of nucleic acids and hybridiza

ISBN	1578083613	DEWEY	572.8
ISBN13	9781578083619 (What's this?)	DEWEY edition	DC22
Publisher	Taylor & Francis Inc	Pages	230
Imprint	Science Publishers,U.S.	Volumes	001
Format	Hardback	Published in	Enfield
Publication date	01 Apr 2005	Height (mm)	250
Library of Congress	QH506.P744	Academic level	Undergraduate, Postgraduate, Professional / Scholarly

I	Definition	1
1	Structure and expression of a eukaryote gene coding for an mRNA and a protein	3
2	Parameters of description of a genome	5
3	Sequencing of whole genomes	9
II	Vectors and cloning	13
4	Restriction enzymes	15
5	Electrophoresis of nucleic acids	19
6	Description of a plasmid and a phagemid	21
7	Description of a bacteriophage and a cosmid	23
8	Description of a YAC and other large vectors	25
9	Molecular cloning	27
10	Genetic transformation of bacteria and yeasts	31
III	Labelling of nucleic acids and hybridization	33
11	DNA labelling	35
12	Molecular hybridization	39
13	In situ hybridization of mRNA	43
IV	DNA libraries and screening	47
14	Construction of a genomic DNA library	49
15	Construction of a cDNA library	51
16	Screening of a library	53
17	Differential screening : subtractive libraries, AFLP-cDNA	57
18	Differential display RT-PCR	61
19	Differential screening by SSH : suppression subtractive hybridization	65
20	Differential screening by RDA : representational difference analysis	69
21	EST : expressed sequence tags	73
	More...

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